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Non-coding RNA Expression, Function, and Variation during Drosophila Embryogenesis
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DOI：10.1016/j.cub.2018.09.026.png)
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摘要
En 中文
Long non-coding RNAs (IncRNAs) can often function in the regulation of gene expression during development; however, their generality as essential regulators in developmental processes and organismal phenotypes remains unclear. Here, we performed a tailored investigation of IncRNA expression and function during Drosophila embryogenesis, interrogating multiple stages, tissue specificity, nuclear localization, and genetic backgrounds. Our results almost double the number of annotated IncRNAs expressed at these embryonic stages. IncRNA levels are generally positively correlated with those of their neighboring genes, with little evidence of transcriptional interference. Using fluorescent in situ hybridization, we report the spatiotemporal expression of 15 new IncRNAs, revealing very dynamic tissue-specific patterns. Despite this, deletion of selected IncRNA genes had no obvious developmental defects or effects on viability under standard and stressed conditions. However, two IncRNA deletions resulted in modest expression changes of a small number of genes, suggesting that they fine-tune expression of non-essential genes. Several IncRNAs have strain-specific expression, indicating that they are not fixed within the population. This intra-species variation across genetic backgrounds may thereby be a useful tool to distinguish rapidly evolving IncRNAs with as yet non-essential roles.
Keyword:
CELL-FATE
TRANSCRIPTIONAL REGULATION
MESSENGER-RNA
X-CHROMOSOME
GENE
EVOLUTION
ENHANCER
DORSOCROSS
LINCRNAS
RECONSTRUCTION
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期刊
IF:
7.5
论文数:
2.2W
被引数:
7.8W
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