.png){kind=icon}
返回
Precise transcript targeting by CRISPR-Csm complexes
.png){kind=icon}
OA
AI
DOI：10.1038/s41587-022-01649-9.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Robust and precise transcript targeting in mammalian cells remains a difficult challenge using existing approaches due to inefficiency, imprecision and subcellular compartmentalization. Here we show that the clustered regularly interspaced short palindromic repeats (CRISPR)-Csm complex, a multiprotein effector from type III CRISPR immune systems in prokaryotes, provides surgical RNA ablation of both nuclear and cytoplasmic transcripts. As part of the most widely occurring CRISPR adaptive immune pathway, CRISPR-Csm uses a programmable RNA-guided mechanism to find and degrade target RNA molecules without inducing indiscriminate trans-cleavage of cellular RNAs, giving it an important advantage over the CRISPR-Cas13 family of enzymes. Using single-vector delivery of the Streptococcus thermophilus Csm complex, we observe high-efficiency RNA knockdown (90-99%) and minimal off-target effects in human cells, outperforming existing technologies including short hairpin RNA- and Cas13-mediated knockdown. We also find that catalytically inactivated Csm achieves specific and durable RNA binding, a property we harness for live-cell RNA imaging. These results establish the feasibility and efficacy of multiprotein CRISPR-Cas effector complexes as RNA-targeting tools in eukaryotes. The bacterial Csm complex efficiently knocks down eukaryotic nuclear and cytoplasmic RNAs.
Keyword:
NUCLEIC-ACID DETECTION
DOUBLE-STRANDED-RNA
CAS SYSTEM
DNA
CLEAVAGE
ENDONUCLEASE
INTERFERENCE
MECHANISMS
INJECTION
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
41.7
论文数:
1.2W
被引数:
10.1W
机构
引用论文
Clarifying missing at random and related definitions, and implications when coupled with exchangeability: Table 1..png)
Biometrika
BiometrikaIF0
PREAI
分享收藏High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and CleavageCELL REPORTSOAAI
CELL REPORTSIF6.9
OAAI分享收藏The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma CellsCRISPR-Cas13a基因编辑系统诱导胶质瘤细胞中RNA的侧支裂解ADVANCED SCIENCEOAAI
ADVANCED SCIENCEIF14.1
OAAI分享收藏Repurposing type I-F CRISPR-Cas system as a transcriptional activation tool in human cellsNATURE COMMUNICATIONSOAAI
NATURE COMMUNICATIONSIF15.7
OAAI分享收藏
查看更多内容