.png){kind=icon}
返回
Rapidly evolving homing CRISPR barcodes
.png){kind=icon}
OA
AI
DOI：10.1038/NMETH.4108.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
We present an approach for engineering evolving DNA barcodes in living cells. A homing guide RNA (hgRNA) scaffold directs the Cas9-h9RNA complex to the DNA locus of the hgRNA itself. We show that this homing CRISPR-Cas9 system acts as an expressed genetic barcode that diversifies its sequence and that the rate of diversification can be controlled in cultured cells. We further evaluate these barcodes in cell populations and show that they can be used to record lineage history and that the barcode RNA can be amplified in situ, a prerequisite for in situ sequencing. This integrated approach will have wide-ranging applications, such as in deep lineage tracing, cellular barcoding, molecular recording, dissecting cancer biology, and connectome mapping.
Keyword:
IN-VIVO
RNA
CELLS
SITU
CAS9
DNA
BIOLOGY
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
32.1
论文数:
7.2K
被引数:
12.7W
机构
引用论文
PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing.png)
NATURE COMMUNICATIONSOAAI
NATURE COMMUNICATIONSIF15.7
OAAI分享收藏ZaphodProceedings of the 7th ACM Workshop on Encrypted Computing & Applied Homomorphic CryptographyOAAI
Proceedings of the 7th ACM Workshop on Encrypted Computing & Applied Homomorphic CryptographyIF0
OAAI分享收藏Tracking single hematopoietic stem cells in vivo using high-throughput sequencing in conjunction with viral genetic barcodingNATURE BIOTECHNOLOGYOAAI
NATURE BIOTECHNOLOGYIF41.7
OAAI分享收藏
查看更多内容