Resolving sequencing-based HIV-1 epitranscriptomics

doi:10.1080/17501911.2025.2504333

2025-05-16

PRE

Michael S. Bosmeny

João I. Mamede

Keith T. Gagnon *

DOI：10.1080/17501911.2025.2504333

原文链接

原文求助

摘要

En 中文

HIV-1 RNA化学修饰的收集及其在病毒基因表达、宿主相互作用和病毒生命周期中的功能后果，被称为HIV-1表观转录组学，目前仍未被完全理解。尽管该领域正在发展，但多样的修饰发现方法、细胞系、HIV-1序列和生物信息学方法使得对HIV-1表观转录组的共识观点难以确立。本文综述了在HIV-1中识别和解释N6-甲基腺苷（m6A）、5-甲基胞嘧啶（m5C）、假尿苷（Psi）、2′-O-甲基化（Nm）和N4-乙酰胞嘧啶（ac4C）修饰的方法，包括抗体介导的选择方法、化学处理介导的选择方法以及通过纳米孔直接RNA测序进行检测。我们建议采用后者作为标准化的测序策略，以促进不同研究间的更好比较，并有助于阐明HIV-1表观转录组学。

Keyword:

HIV-1

epitranscriptomics

nanopore

sequencing

RNA modification

期刊

Epigenomics

IF:

2.6

论文数:

1.8K

被引数:

3.4K

机构

暂无机构信息

引用论文

Structural Differentiation of the HIV-1 Poly(A) Signals

Journal of Biomolecular Structure and Dynamics

IF0

2006-02-01

OAAI

Alan H. Gee; Wojciech Kasprzak; Bruce A. Shapiro

Mapping RNA Modifications Using Photo-Crosslinking-Assisted Modification Sequencing

Methods in Molecular Biology

IF0

2021-06-04

PREAI

Bryan R. Cullen; Kevin Tsai

Post-transcriptional pseudouridylation in mRNA as well as in some major types of noncoding RNAs

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms

IF0

2019-03-01

OAAI

Hironori Adachi; Meemanage D. De Zoysa; Yi-Tao Yu

Ultrafast bisulfite sequencing detection of 5-methylcytosine in DNA and RNA DNA和RNA中5-甲基胞嘧啶的超快亚硫酸氢盐测序检测

NATURE BIOTECHNOLOGY

IF41.7

2024-01-02

OAAI

Dai, Qing; Ye, Chang; Irkliyenko, Iryna; Wang, Yiding; Sun, Hui-Lung; Gao, Yun; Liu, Yushuai; Beadell, Alana; Perea, Jose; Goel, Ajay; He, Chuan

Stabilization of RNA stacking by pseudouridine

Nucleic Acids Research

IF0

1995-01-01

OAAI

Darrell R. Davis

Positive-sense RNA viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection

NUCLEIC ACIDS RESEARCH

IF13.1

2018-01-24

114

OAAI

McIntyre, Will; Netzband, Rachel; Bonenfant, Gaston; Biegel, Jason M.; Miller, Clare; Fuchs, Gabriele; Henderson, Eric; Arra, Manoj; Canki, Mario; Fabris, Daniele; Pager, Cara T.

Making the Mark: The Role of Adenosine Modifications in the Life Cycle of RNA Viruses

Cell Host & Microbe

IF0

2017-06-01

OAAI

Sarah R. Gonzales-van Horn; Peter Sarnow

De novoIdentification of DNA Modifications Enabled by Genome-Guided Nanopore Signal Processing

IF0

2016-12-15