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RNA-Seq: a revolutionary tool for transcriptomics
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DOI：10.1038/nrg2484.png)
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RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.
Keyword:
LARGE-SCALE ANALYSIS
GENE-EXPRESSION
YEAST GENOME
CAP ANALYSIS
DISCOVERY
IDENTIFICATION
MAPS
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4.0K
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