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Self-supervised deep-learning two-photon microscopy

delete2022-12-14
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PRE
AI
何
何悦之 (Yuezhi He)
J
Jing Yao
刘
刘莉娜 (Lina Liu)
Y
Yufeng Gao
J
Jia Yu
S
Shiwei Ye
李慧 封面图
李慧 (Hui Li)
郑炜 封面图
郑炜 (Wei Zheng) *
DOI:10.1364/PRJ.469231delete
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摘要

摘要

En 中文
Artificial neural networks have shown great proficiency in transforming low-resolution microscopic images into high-resolution images. However, training data remains a challenge, as large-scale open-source databases of mi-croscopic images are rare, particularly 3D data. Moreover, the long training times and the need for expensive computational resources have become a burden to the research community. We introduced a deep-learning-based self-supervised volumetric imaging approach, which we termed Self-Vision. The self-supervised approach re-quires no training data, apart from the input image itself. The lightweight network takes just minutes to train and has demonstrated resolution-enhancing power on par with or better than that of a number of recent microscopy -based models. Moreover, the high throughput power of the network enables large image inference with less post -processing, facilitating a large field-of-view (2.45 mm x 2.45 mm) using a home-built two-photon microscopy system. Self-Vision can recover images from fourfold undersampled inputs in the lateral and axial dimensions, dramatically reducing the acquisition time. Self-Vision facilitates the use of a deep neural network for 3D micros-copy imaging, easing the demanding process of image acquisition and network training for current resolution -enhancing networks. (c) 2022 Chinese Laser Press

期刊

Photonics Research 封面图
Photonics Research
IF:
7.2
论文数:
2.4K
被引数:
1.3W

机构

C
chinese academy of sciences
学者数:
56.7W
论文数: 45.0W
被引数: 704
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