.png){kind=icon}
返回
Single cell transcriptomics: moving towards multi-omics
.png){kind=icon}
PRE
AI
DOI：10.1039/c8an01852a.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
As the basic units of life, cells present dramatic heterogeneity which, although crucial to an organism's behavior, is undetected by bulk analysis. Recently, much attention has been paid to reveal cellular types and states at the single-cell level including genome, transcriptome, epigenome or proteome-based on sequencing and immunological methods, etc. Among these approaches, transcriptomic analysis provides knowledge of the molecular linkages between genetic information and the proteome, leading to a comprehensive understanding of biological processes and diseases. Compared to single-dimensional inspection, multi-dimensional analysis combines the transcriptome with other omics to enable a comprehensive understanding of single-cell processes and functions. Moreover, compared to separate observations or single snapshots, spatial dimension and lineage time tracing can provide a more multifaceted understanding of the micro-environment and dynamic processes. In this review, we will introduce current transcriptomic analysis methods, as well as their combination with other omics methods including genomic, proteomic, and epigenetic approaches. Multi-dimensional analysis using these approaches for spatial positioning and lineage tracing applications will be reviewed. The future perspectives of single-cell multi-omics analysis based on the transcriptome will also be discussed.
Keyword:
NUCLEUS RNA-SEQ
EMBRYONIC STEM-CELLS
MESSENGER-RNA
CHROMATIN ACCESSIBILITY
DNA METHYLATION
GENE-EXPRESSION
HIGH-THROUGHPUT
WHOLE-GENOME
DISPLACEMENT AMPLIFICATION
METHYLOME LANDSCAPES
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
A
IF:
3.3
论文数:
1.6W
被引数:
3.3W
机构
引用论文
Smart-seq2 for sensitive full-length transcriptome profiling in single cells单细胞中敏感的全长转录组分析的Smart-seq2.png)
NATURE METHODS
NATURE METHODSIF32.1
PREAI
分享收藏Separation and parallel sequencing of the genomes and transcriptomes of single cells using G&T-seqNATURE PROTOCOLS
NATURE PROTOCOLSIF16
PREAI
分享收藏Control of an atomic force microscopy probe during nano-manipulation via the sliding mode method通过滑模方法在纳米操作过程中控制原子力显微镜探针
OAAI
OAAI分享收藏Determination of iron in seawater by flow injection analysis using in-line preconcentration and spectrophotometric detection在线预富集和分光光度检测通过流动注射分析测定海水中的铁
PREAI
分享收藏
查看更多内容