.png){kind=icon}
返回
Single-molecule mechanical identification and sequencing
.png){kind=icon}
OA
AI
DOI：10.1038/NMETH.1925.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
High-throughput, low-cost DNA sequencing has emerged as one of the challenges of the postgenomic era. Here we present the proof of concept for a single-molecule platform that allows DNA identification and sequencing. In contrast to most present methods, our scheme is not based on the detection of the fluorescent nucleotides but on DNA hairpin length. By pulling on magnetic beads tethered by a DNA hairpin to the surface, the molecule can be unzipped. In this open state it can hybridize with complementary oligonucleotides, which transiently block the hairpin rezipping when the pulling force is reduced. By measuring from the surface to the bead of a blocked hairpin, one can determine the position of the hybrid along the molecule with nearly single-base precision. Our approach can be used to identify a DNA fragment of known sequence in a mix of various fragments and to sequence an unknown DNA fragment by hybridization or ligation.
Keyword:
MAGNETIC TWEEZERS
HUMAN GENOME
DNA
HYBRIDIZATION
INFORMATION
POLYMERASE
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
32.1
论文数:
7.2K
被引数:
12.7W
机构
引用论文
分享收藏分享收藏分享收藏Magnetic tweezers: Micromanipulation and force measurement at the molecular level.png)
BIOPHYSICAL JOURNALOAAI
BIOPHYSICAL JOURNALIF3.1
OAAI分享收藏
查看更多内容