.png){kind=icon}
返回
Single-stranded microRNA mimics
.png){kind=icon}
OA
AI
DOI：10.1261/rna.031278.111.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
miRNAs are similar to 22-nt RNAs that bind to the Argonaute family of proteins and have important regulatory roles in plants and animals. Here, we show that miRNAs exhibit targeting activity in cells when delivered as single strands that are 5'-phosphorylated and that contain 2'-fluoro ribose modifications. Length preferences, chemical modification sensitivity, and genome-wide seed-based targeting all suggest that this activity is Ago-based. Activity could be enhanced by annealing of segmented passenger strands containing non-nucleic acid spacers. Furthermore, screening of randomly generated sequences identified pyrimidine rich 3' cassette sequences that increased single strand activity. These results provide an initial step in the development of single-stranded miRNA mimics for therapeutic use.
Keyword:
miR-122
miR-124
microRNA
mimetic
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
5
论文数:
4.9K
被引数:
1.3W
机构
暂无机构信息
引用论文
Improved silencing properties using small internally segmented interfering RNAs.png)
NUCLEIC ACIDS RESEARCHOAAI
NUCLEIC ACIDS RESEARCHIF13.1
OAAI分享收藏分享收藏Mipomersen, an apolipoprotein B synthesis inhibitor, for lowering of LDL cholesterol concentrations in patients with homozygous familial hypercholesterolaemia: a randomised, double-blind, placebo-controlled trialLANCET
LANCETIF88.5
PREAI
分享收藏Sex trade in a male-to-female transgender population: psychosocial correlates of inconsistent condom use男女跨性别人群中的性交易: 不一致使用安全套的心理社会相关因素
PREAI
分享收藏
查看更多内容