Spatial transcriptomic applications in orthopedics

doi:10.1080/03008207.2025.2501703

2025-05-10

PRE

Thomas L. Jenkins *

Jasper H. N. Yik *

Dominik R. Haudenschild *

DOI：10.1080/03008207.2025.2501703

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摘要

En 中文

目的：本综述强调了空间转录组学对骨科研究的变革性影响，重点介绍了其在解读肌肉骨骼组织内复杂基因表达模式中的应用。方法：本文综述了空间转录组学方法的相关文献，特别是用于肌肉骨骼组织（软骨、关节、骨骼、肌腱、韧带和滑膜）的10X Visium、10X Xenium、seqFISH+、MERFISH和NanoString GeoMx DSP。结果：检索到29篇已发表的论文报道了软骨、骨骼、肌腱、滑膜和椎间盘的空间转录组学数据。大多数关于空间转录组学数据的出版物来自肌腱和滑膜。在已识别的29篇论文中，有22篇使用了10X Visium。空间转录组学已被用于识别调节发育和疾病的全新细胞群体及细胞信号通路。结论：基于成像的空间转录组学方法可能更适合假设检验，因为它们通常具有亚细胞分辨率但测序的基因较少。测序方法可能更适合无靶向的、shotgun方法，能够从完整的组织切片中生成有用的假设。空间转录组学方法可能对临床诊断和精准医学方法具有应用价值。

Keyword:

Spatial transcriptomics

cartilage

orthopedics

arthritis

synovium

期刊

Connective Tissue Research

IF:

2.1

论文数:

102

被引数:

2.6K

机构

Houston Methodist Research Institute

学者数:

294

论文数: 115

被引数: 0

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