STED microscopy with continuous wave beams

Katrin I. Willig

doi:10.1038/NMETH1108

2007-10-21

457

PRE

DOI：10.1038/NMETH1108

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摘要

En 中文

We report stimulated emission depletion (STED) fluorescence microscopy with continuous wave (CW) laser beams. Lateral fluorescence confinement from the scanning focal spot delivered a resolution of 29 - 60 nm in the focal plane, corresponding to a 5 - 8- fold improvement over the diffraction barrier. Axial spot confinement increased the axial resolution by 3.5-fold. We observed three-dimensional (3D) subdiffraction resolution in 3D image stacks. Viable for fluorophores with low triplet yield, the use of CW light sources greatly simplifies the implementation of this concept of far-field fluorescence nanoscopy.

Keyword:

FLUORESCENCE MICROSCOPY

STIMULATED-EMISSION

RESOLUTION

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期刊

Nature Methods

IF:

32.1

论文数:

7.2K

被引数:

12.7W

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