Super-resolution microscopy demystified

Lothar Schermelleh

doi:10.1038/s41556-018-0251-8

2019-01-02

806

PRE

Gregor P. C. Drummen *

DOI：10.1038/s41556-018-0251-8

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摘要

En 中文

Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.

Keyword:

STRUCTURED-ILLUMINATION MICROSCOPY

RESOLUTION FLUORESCENCE MICROSCOPY

EXPANSION MICROSCOPY

LIVE CELLS

LOCALIZATION MICROSCOPY

CELLULAR STRUCTURES

DIFFRACTION LIMIT

IMAGE-RESOLUTION

STED NANOSCOPY

DNA ORIGAMI

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Nature Cell Biology

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