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Super-Resolved Traction Force Microscopy (STFM)
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DOI：10.1021/acs.nanolett.6b00273.png)
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摘要
En 中文
Measuring small forces is a major challenge in cell biology. Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM) using STED microscopy. The increased spatial resolution of STED-TFM (STFM) allows a greater than 5-fold higher sampling of the forces generated by the cell than conventional TFM, accessing the nano instead of the micron scale. This improvement is highlighted by computer simulations and an activating RBL cell model system.
Keyword:
Super-resolution microscopy
traction force microscopy
mechanobiology
actin cytoskeleton
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期刊
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9.1
论文数:
2.7W
被引数:
16.5W
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