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Synthetic Amine Linkers for Efficient Sortagging
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DOI：10.1021/acs.bioconjchem.4c00143.png)
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摘要
En 中文
Enzymatic site-specific bioconjugation techniques, in particular sortase-mediated ligation, are increasingly used to generate conjugated proteins for a wide array of applications. Extension of the utility and practicality of sortagging for diverse purposes is critically dependent on further improvement of the efficiency of sortagging reactions with a wider structural variety of substrates. We present a comprehensive comparative mass spectrometry screening study of synthetic nonpeptidic incoming amine nucleophile substrates of Staphylococcus aureus Sortase A enzyme. We have identified the optimal structural motifs among the chemically diverse set of 452 model primary and secondary amine-containing sortagging substrates, and we demonstrate the utility of representative amine linkers for efficient C-terminal biotinylation of nanobodies.
Keyword:
MEDIATED BIOCONJUGATION
SORTASE
PROTEINS
LIGATION
NUCLEOPHILES
ANTIBODIES
期刊
IF:
3.9
论文数:
7.2K
被引数:
1.6W
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