.png){kind=icon}
返回
Targeting cells with single vectors using multiple-feature Boolean logic
.png){kind=icon}
OA
AI
DOI：10.1038/NMETH.2996.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Precisely defining the roles of specific cell types is an intriguing frontier in the study of intact biological systems and has stimulated the rapid development of genetically encoded tools for observation and control. However, targeting these tools with adequate specificity remains challenging: most cell types are best defined by the intersection of two or more features such as active promoter elements, location and connectivity. Here we have combined engineered introns with specific recombinases to achieve expression of genetically encoded tools that is conditional upon multiple cell-type features, using Boolean logical operations all governed by a single versatile vector. We used this approach to target intersectionally specified populations of inhibitory interneurons in mammalian hippocampus and neurons of the ventral tegmental area defined by both genetic and wiring properties. This flexible and modular approach may expand the application of genetically encoded interventional and observational tools for intact-systems biology.
Keyword:
IN-VIVO
EXPRESSION CASSETTES
MAMMALIAN-CELLS
GENE-EXPRESSION
NEURAL CIRCUITS
NEURONS
BRAIN
RECOMBINATION
HIPPOCAMPUS
AREA
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
32.1
论文数:
7.2K
被引数:
12.7W
机构
引用论文
US incidence of juvenile dermatomyositis, 1995–1998: Results from the National Institute of Arthritis and Musculoskeletal and Skin Diseases Registry美国青少年皮肌炎发病率，1995-1998: 来自美国国家关节炎和肌肉骨骼和皮肤疾病登记处的结果
PREAI
分享收藏In vitro and in vivo gene therapy vector evolution via multispecies interbreeding and retargeting of adeno-associated viruses.png)
JOURNAL OF VIROLOGYOAAI
JOURNAL OF VIROLOGYIF3.8
OAAI分享收藏Concatamerization of adeno-associated virus circular genomes occurs through intermolecular recombinationJOURNAL OF VIROLOGYOAAI
JOURNAL OF VIROLOGYIF3.8
OAAI分享收藏Steel Parts with Tailored Material Gradients by 3D-Printing Using Nano-Particulate Ink使用纳米颗粒墨水通过3d打印具有定制材料梯度的钢制零件
PREAI
分享收藏Increasing the size of rAAV-mediated expression cassettes in vivo by intermolecular joining of two complementary vectorsNATURE BIOTECHNOLOGY
NATURE BIOTECHNOLOGYIF41.7
PREAI
分享收藏Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures神经回路的光遗传学询问: 探测哺乳动物大脑结构的技术NATURE PROTOCOLSOAAI
NATURE PROTOCOLSIF16
OAAI分享收藏
查看更多内容