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The Third Revolution in Sequencing Technology
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DOI：10.1016/j.tig.2018.05.008.png)
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Forty years ago the advent of Sanger sequencing was revolutionary as it allowed complete genome sequences to be deciphered for the first time. A second revolution came when next-generation sequencing (NGS) technologies appeared, which made genome sequencing much cheaper and faster. However, NGS methods have several drawbacks and pitfalls, most notably their short reads. Recently, third-generation/long-read methods appeared, which can produce genome assemblies of unprecedented quality. Moreover, these technologies can directly detect epigenetic modifications on native DNA and allow whole-transcript sequencing without the need for assembly. This marks the third revolution in sequencing technology. Here we review and compare the various long-read methods. We discuss their applications and their respective strengths and weaknesses and provide future perspectives.
Keyword:
SINGLE-MOLECULE
REAL-TIME
DNA METHYLATION
HUMAN GENOME
NANOPORE
TRANSCRIPTOME
N-6-ADENINE
COMPLEXITY
RESOLUTION
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Direct detection of DNA methylation during single-molecule, real-time sequencing.png)
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