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Tomographic phase microscopy
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DOI：10.1038/NMETH1078.png)
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We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.
Keyword:
DIGITAL HOLOGRAPHIC MICROSCOPY
REFRACTIVE-INDEX
RESOLUTION
CELLS
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32.1
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7.2K
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分享收藏Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopyOPTICS EXPRESSOAAI
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