.png){kind=icon}
返回
Two-photon PSF-engineered image scanning microscopy
.png){kind=icon}
OA
AI
DOI：10.1364/OL.44.000895.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
We present two-photon fluorescence image scanning microscopy (ISM) with engineered excitation and detection point-spread-functions enabling 3D imaging in a single 2D scan. This demonstration combines excitation using a holographic multispot array of focused femtosecond pulses with a high-effidency single-helix PSF phase mask detection. Camera detection along with a multiview reconstruction algorithm allows volumetric imaging of biological samples over a depth of field spanning more than 1500 nm with an axial resolution of better than 400 nm. The nonlinear two-photon process improves sectioning and the inherent longer wavelengths increase the penetration depth in scattering samples. Our method extends the performance of 3D ISM towards thicker biological samples. (C) 2019 Optical Society of America
Keyword:
STRUCTURED ILLUMINATION MICROSCOPY
POINT-SPREAD FUNCTION
SUPERRESOLUTION
DECONVOLUTION
EXCITATION
IMPROVES
期刊
IF:
3.3
论文数:
4.0W
被引数:
7.6W
机构
引用论文
A technical review on gas diffusion, mechanism and medium of PEM fuel cellPEM燃料电池气体扩散、机理及介质技术综述.png)
Ionics
IonicsIF0
PREAI
分享收藏Three-dimensional information from two-dimensional scans: a scanning microscope with postacquisition refocusing capabilityOPTICA
OAAI
OPTICAIF8.5
OAAI分享收藏Deconvolution approach for 3D scanning microscopy with helical phase engineeringOPTICS EXPRESSOAAI
OPTICS EXPRESSIF3.3
OAAI分享收藏Urinary uromodulin independently predicts end-stage renal disease and rapid kidney function decline in a cohort of chronic kidney disease patientsMedicineOAAI
MedicineIF0
OAAI分享收藏分享收藏
查看更多内容