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A sentinel protein assay for simultaneously quantifying cellular processes

delete2014-09-07
delete57
PRE
AI
M
Martin Soste
R
Rita Hrabáková
S
Stefanie Wanka
A
André Melnik
P
Paul J. Boersema
A
Alessio Maiolica
T
Timon Wernas
M
Marco Tognetti
C
Christian von Mering
P
Paola Picotti *
DOI：10.1038/NMETH.3101delete
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Abstract

Abstract

En 中文
We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in-high throughput.
Keywords:
SACCHAROMYCES-CEREVISIAE
HEAT-SHOCK
KINASE-A
YEAST
PHOSPHORYLATION
PROTEOMICS
REVEALS
RAPAMYCIN
DYNAMICS
STRESS
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Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.

Journal

Nature Methods cover
Nature Methods
IF:
32.1
Papers:
7.2K
Citations:
12.7W

Organization

U
university of zurich
Scholars:
5.0W
Papers: 4.0W
Citations: 65
C
czech academy of sciences
Scholars:
3.4W
Papers: 2.6W
Citations: 31
S
swiss federal institutes of technology domain
Scholars:
9.0W
Papers: 8.0W
Citations: 163
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