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An improved surface passivation method for single-molecule studies
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DOI：10.1038/NMETH.3143.png)
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Abstract
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We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly (ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.
Keywords:
IN-VITRO
PROTEINS
HELICASE
DNA
INITIATION
DYNAMICS
COMPLEX
MYOSIN
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