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Enzymatic profiling system in a small-molecule microarray
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DOI：10.1021/ol034233h.png)
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Abstract
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We have developed a microarray-based strategy for detection of three major classes of hydrolytic enzymes on the basis of their catalytic activities. This enables the sensitive detection of proteins not merely by their bindings but rather by their enzymatic activities. This may provide a valuable tool for screening, identification, and characterization of new enzymes in a high-throughput fashion.
Keywords:
PROTEIN FUNCTION
KINASE-ACTIVITY
STRATEGIES
ARRAYS
CHIPS
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