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Genetic code expansion for multiprotein complex engineering
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DOI：10.1038/NMETH.4032.png)
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Abstract
En 中文
We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and robust protein production platform, 'MultiBacTAG', (i) for the fluorescent labeling of target proteins and biologics using click chemistries, (ii) for glycoengineering of antibodies, and (iii) for structure-function studies of novel eukaryotic complexes using single-molecule Forster resonance energy transfer as well as site-specific crosslinking strategies.
Keywords:
UNNATURAL AMINO-ACIDS
MAMMALIAN-CELLS
SINGLE-MOLECULE
DROSOPHILA-MELANOGASTER
PROTEIN
SYSTEM
RECOMMENDATIONS
FLUORESCENCE
EXPRESSION
TFIIA
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