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High-throughput functional genomics using CRISPR-Cas9
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DOI：10.1038/nrg3899.png)
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Abstract
En 中文
Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity. We discuss practical aspects of screen design, provide comparisons with RNA interference (RNAi) screening, and outline future applications and challenges.
Keywords:
SEQUENCE-SPECIFIC CONTROL
LENTIVIRAL RNAI LIBRARY
CANCER GENE DISCOVERY
DOUBLE-STRANDED-RNA
HUMAN-CELLS
IN-VIVO
SHRNA LIBRARIES
TARGET DNA
CAENORHABDITIS-ELEGANS
NUCLEASE SPECIFICITY
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