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High Throughput Screening and Selection Methods for Directed Enzyme Evolution
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DOI：10.1021/ie503060a.png)
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Abstract
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Successful evolutionary enzyme engineering requires a high throughput screening or selection method, which considerably increases the chance of obtaining desired properties and reduces the time and cost. In this review, a series of high throughput screening and selection methods are illustrated With significant and recent examples. These high throughput strategies are also discussed with an emphasis on compatibility with phenotypic analysis during directed enzyme evolution. Lastly, certain limitations of current methods, as well as future developments, are briefly summarized.
Keywords:
IN-VITRO SELECTION
RESONANCE ENERGY-TRANSFER
CELL-SURFACE DISPLAY
RIBOSOME DISPLAY
PHAGE DISPLAY
PROTEIN
SYSTEM
IDENTIFICATION
BIOCATALYSTS
SPECIFICITY
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