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Isotope-Coded Labeling for Accelerated Protein Interaction Profiling Using MS
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DOI：10.1021/acs.analchem.5b01410.png)
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Abstract
En 中文
Protein interaction surface mapping using MS is widely applied but comparatively resource-intensive. Here, a workflow adaptation for use of isotope-coded tandem mass tags for the purpose is reported. The key benefit of improved throughput derived from sample acquisition multiplexing and automated analysis is shown to be maintained in the new application. Mapping of the epitopes of two monoclonal antibodies on their respective targets serves to illustrate the novel approach. We conclude that the approach enables mapping of interactions by MS at significantly larger scales than hereto possible.
Keywords:
EXCHANGE-MASS-SPECTROMETRY
CHEMICAL CROSS-LINKING
FAST PHOTOCHEMICAL OXIDATION
HYDROGEN/DEUTERIUM EXCHANGE
HYDROGEN-EXCHANGE
STRUCTURAL PROTEOMICS
SURFACE DISPLAY
RADICAL PROBE
COMPLEX
DIETHYLPYROCARBONATE
Journal
IF:
6.7
Papers:
4.7W
Citations:
15.9W
Organization
Cited Papers
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METHODS
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