Metabolomic profiling and chemometric analysis for metabolite authentication in processed meats

2026-05-23

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Laffranchi, Mattia *

Zhang, Wenhao

Putri Widyanti Harlina

Muchtaridi Muchtaridi

Nor Kartini Abu Bakar

A. Rohman

Dachriyanus Dachriyanus

Nur Kusaira Khairul Ikram

Anjar Windarsih

Mohamad Rafi

DOI：10.1080/10942912.2026.2639766

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Abstract

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This study aims to authenticate the halal status of beef and pork products through a combination of metabolomics and chemometric approaches. The methods consisted of metabolite extraction using methanol, metabolomic analysis using UHPLC-HRMS (Ultra High-Performance Liquid Chromatography-High Resolution Mass Spectrometry), and chemometric analysis (PCA, cluster analysis, and PLS-DA). The results showed that 86 metabolites were identified with creatine, creatinine, and DL carnitine as metabolites with the highest levels area (1074640583, 2,560,861,540, and 272,005,238). PCA grouped beef and pork processed products well. Cluster analysis using dendrogram and SOM (Self-Organizing Map) showed the clustering of beef and pork products. The PLS-DA results showed 15 potential metabolites that could be markers for authentication of halal status of beef and pork products DL-Malic acid, myristyl sulfate, sorbic acid, L-Pyroglutamic, hypoxanthine, taurine, (9Z)-9-Octadecenamide, acetylcarnitine, DL-histidine, daidzein, citric acid, phytosphingosine, propiomylcarnitine, 6-O-Phosphonohex-2-ulofuranose, and D-sucrose. The R2 value in the PLS-DA analysis is 0.952 and Q2 value 0.616, showing that the analysis results are accurate. The combined metabolic and chemometric approach is accurate analytical method for authenticating of beef and pork products.

Keywords:

Meat products

pork products

metabolite

authentication

chemometrics

Metabolomic profiling and chemometric analysis for metabolite authentication in processed meats

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