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Multicolor imaging based on brightness coded set
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DOI：10.1016/j.optlaseng.2024.108552.png)
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Abstract
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Fluorescence imaging necessitates precise matching of excitation source, dichroic mirror, emission filter, detector and dyes, which is complex and time-consuming, especially for applications of probe multiplexing. We propose a novel method for multicolor imaging based on a brightness coded set. Each brightness code consists of 12 bits (OOOXXXYYYZTT), denoting probe type, cube, emission filter, imaging result and priority, respectively. The brightness of a probe in an imaging system is defined as the product of extinction coefficient, quantum yield and the filter transmittance. When the brightness exceeds the threshold, Z=1 indicates a clear image, otherwise Z=0. The higher the brightness value the higher the priority (TT). To validate the efficacy and efficiency of the coding method, we conducted two separate experiments involving four-color imaging. The proposed method offers a substantial simplification of the conventional approach to device matching in multicolor imaging by leveraging spectrograms, and presents a promising avenue for the advancement of intelligent multicolor imaging systems.
Keywords:
Multicolor imaging
Fluorescence microscopy
Brightness coded
Filter set
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