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PASTA: versatile tyramide-oligonucleotide amplification for multimodal spatial biology
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DOI：10.1038/s41592-026-03200-z.png)
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Abstract
En 中文
Spatial proteomics is limited by detection sensitivity, multiplexing and multimodal integration, leaving a gap between discovery and clinical assays. Here we present protein and nucleic acid serial tyramide amplification (PASTA), using horseradish peroxidase-mediated oligonucleotide deposition and cyclical imaging for high-plex, multimodal spatial profiling. Compatible with conjugated antibodies and in situ hybridization, PASTA enables simultaneous protein and RNA codetection from formalin-fixed, paraffin-embedded samples, providing a cost-effective bridge from discovery to clinical validation. PASTA is a platform-agnostic signal amplification approach for multiplexed imaging across modalities.
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