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Super-resolution re-scan second harmonic generation microscopy
DOI:10.1073/pnas.2214662119.png)
Abstract
En 中文
Second harmonic generation microscopy (SHG) is generally acknowledged as a powerful tool for the label-free three-dimensional visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Despite the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we demonstrate super-resolved re-scan SHG, qualitatively and quantitatively showing on collagenous tissues the available resolution advantage over the diffraction limit. We introduce as well super-resolved re-scan two-photon excited fluorescence microscopy, an imaging modality not explored to date.
Keywords:
second harmonic generation
two-photon excited fluorescence
super-resolved optical imaging
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Journal
P
IF:
9.1
Papers:
10.8W
Citations:
73.5W
Organization
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