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Super-resolution re-scan second harmonic generation microscopy

delete2022-11-14
delete15
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OA
AI
S
Stefan G. Stanciu *
R
Radu Hristu
G
George A. Stanciu
D
Denis E. Tranca
L
Lucian G. Eftimie
A
Adrian Dumitru
M
Mariana Costache
H
Harald Stenmark
H
Harm Manders
A
Amit Cherian
M
Mariliis Tark‐Dame
E
Erik M. M. Manders
DOI:10.1073/pnas.2214662119delete
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Abstract

Abstract

En 中文
Second harmonic generation microscopy (SHG) is generally acknowledged as a powerful tool for the label-free three-dimensional visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Despite the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we demonstrate super-resolved re-scan SHG, qualitatively and quantitatively showing on collagenous tissues the available resolution advantage over the diffraction limit. We introduce as well super-resolved re-scan two-photon excited fluorescence microscopy, an imaging modality not explored to date.
Keywords:
second harmonic generation
two-photon excited fluorescence
super-resolved optical imaging
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Journal

P
Proceedings of the National Academy of Sciences of the United States of America
IF:
9.1
Papers:
10.8W
Citations:
73.5W

Organization

C
carol davila university of medicine & pharmacy
Scholars:
8.6K
Papers: 4.6K
Citations: 13
U
university of oslo
Scholars:
4.2W
Papers: 3.5W
Citations: 53
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Cited Papers

Cited Papers

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