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The invertor knock-in conditional chromosomal translocation mimic

delete2004-12-21
delete35
PRE
AI
A
A. Förster
R
Richard Pannell
D
Drynan, LF
R
Rosalind Codrington
A
Angelika Daser
M
Markus Metzler
M
Mark N. Lobato
T
Terence H. Rabbitts
DOI：10.1038/NMETH727delete
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Abstract

Abstract

En 中文
Knock-in models of tumor-specific chromosomal translocations can generate Lethal mutations. To circumvent this, a new conditional gene fusion model has been developed (invertor mice) and exemplified with the Ews-ERG fusion oncogene. An ERG segment, flanked by loxP sites, was knocked in to an intron of the Ews gene but in an inverted transcription orientation and lineage-specific Ews-ERG fusion created by Cre-mediated inversion. This invertor method is a completely conditional approach, applicable to any gene fusion, to emulate effects of translocations found in human cancers.
Keywords:
HUMAN CANCER
CRE-LOXP
RECOMBINATION
GENE
FUSION
MOUSE
LIPOSARCOMA
ONCOGENE
LEUKEMIA
CELLS
AI Summary

AI Summary

Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.

Journal

Nature Methods cover
Nature Methods
IF:
32.1
Papers:
7.2K
Citations:
12.7W

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