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L
Liang Tong
H指数:91
被引数:2.6万
个人简介
A major focus of our research is on proteins involved in pre-mRNA and non-coding RNA 3'-end processing. Most eukaryotic mRNA precursors must undergo cleavage and polyadenylation in their 3'-ends before they can function as mRNAs. This 1.6 MDa canonical processing machinery contains more than 16 protein factors. Replication-dependent histone pre-mRNAs are cleaved at their 3'-ends but not polyadenylated, and a distinct machinery, U7 snRNP (1 MDa), is required for this processing. Integrator (17 subunits, 1.6 MDa) is involved in non-coding RNA 3'-end processing and attenuation of pre-mRNA expression through promoter-proximal transcription termination of RNA polymerase II. The goal of our research is to understand the molecular basis of these important cellular events. We will produce structures of the protein subunits, protein-protein and protein-RNA sub-complexes, and the entire machinery, and carry out functional studies to assess the structural information. Our recent studies unexpectedly led to the identification of an mRNA 5'-end capping quality control mechanism. We have identified the enzymes (Rai1, Dxo1, DXO) that play a central role in this mechanism. We are characterizing their biochemical properties and deciphering their physiological functions in yeast as well as mammalian cells. Another area of our research is on enzymes that are involved in fatty acid and/or carbohydrate metabolism. These include acetyl-coenzyme A carboxylase (ACC), carnitine acyltransferase, AMP-activated protein kinase (AMPK), ATP-citrate lyase (ACLY) and others. These enzymes are important targets for drug discovery against obesity, diabetes and other human diseases. The goals of our research are to produce structural information on these enzymes and to understand their functions at the molecular level. The structural information will also lay the foundation for drug discovery against these targets.
教育背景
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工作经历
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荣誉奖项
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机制选择性的两亲性树状化刷状嵌段共聚物自组装成层状和多孔光子颜料
小鼠DXO与UDP-N-乙酰葡糖胺帽复合物的晶体结构及脱帽反应的分子机制
Nucleic Acids Research
IF13.1
SUMO2通过稳定组蛋白基因座体相互作用并促进U7 snRNP的组装来促进组蛋白前体mRNA的加工。
GENES & DEVELOPMENT
IF7.7
低频 polyadenylation signals 被 mPSF 识别的分子基础
NUCLEIC ACIDS RESEARCH
IF13.1
针灸调节微生物-肠-脑轴：一种治疗帕金森病的新策略
大尺寸锂离子电池中由温度不均匀性引起的异质锂沉积的定量研究
人类rixosome整体结构的分子洞察
NATURE COMMUNICATIONS
IF15.7
船用质子交换膜燃料电池动态性能分析
ENERGY
IF9.4
基于观测器的燃料电池系统空气流量和压力解耦控制
MBIO
IF4.7
MOLECULAR CELL
IF16.6
抗癌苯并氧硼唑通过直接抑制CPSF3阻断前mRNA加工
短壁块充填开采矸石充填材料中重金属离子迁移机理及控制技术
