.png){kind=icon}
返回
Computational methods for transcriptome annotation and quantification using RNA-seq
.png){kind=icon}
PRE
AI
DOI：10.1038/NMETH.1613.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
High-throughput RNA sequencing (RNA-seq) promises a comprehensive picture of the transcriptome, allowing for the complete annotation and quantification of all genes and their isoforms across samples. Realizing this promise requires increasingly complex computational methods. These computational challenges fall into three main categories: (i) read mapping, (ii) transcriptome reconstruction and (iii) expression quantification. Here we explain the major conceptual and practical challenges, and the general classes of solutions for each category. Finally, we highlight the interdependence between these categories and discuss the benefits for different biological applications.
Keyword:
DIFFERENTIAL EXPRESSION ANALYSIS
MESSENGER-RNA
SPLICE JUNCTIONS
ALIGNMENT
GENOME
REVEALS
TOOL
NORMALIZATION
CONSTRUCTION
LANDSCAPE
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
32.1
论文数:
7.3K
被引数:
12.7W
机构
引用论文
Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for catalytic activity.png)
Gene
GeneIF0
PREAI
分享收藏Use of HCV‐infected organs in solid organ transplantation: An ethical challenge but plausible option
PREAI
分享收藏Extended Core Sequences from the cHS4 Insulator Are Necessary for Protecting Retroviral Vectors from Silencing Position Effects来自cHS4绝缘子的扩展核心序列对于保护逆转录病毒载体免受沉默位置效应是必需的
PREAI
分享收藏A Fixed-Point Neural Network Architecture for Speech Applications on Resource Constrained Hardware用于资源受限硬件上语音应用的定点神经网络体系结构
PREAI
分享收藏Significance analysis of microarrays applied to the ionizing radiation response微阵列应用于电离辐射响应的意义分析
OAAI
OAAI分享收藏分享收藏
查看更多内容