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CRISPR-SKIP: programmable gene splicing with single base editors
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DOI：10.1186/s13059-018-1482-5.png)
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摘要
En 中文
CRISPR gene editing has revolutionized biomedicine and biotechnology by providing a simple means to engineer genes through targeted double-strand breaks in the genomic DNA of living cells. However, given the stochasticity of cellular DNA repair mechanisms and the potential for off-target mutations, technologies capable of introducing targeted changes with increased precision, such as single-base editors, are preferred. We present a versatile method termed CRISPR-SKIP that utilizes cytidine deaminase single-base editors to program exon skipping by mutating target DNA bases within splice acceptor sites. Given its simplicity and precision, CRISPR-SKIP will be broadly applicable in gene therapy and synthetic biology.
Keyword:
Gene editing
Base editing
Exon skipping
Alternative splicing
Synthetic biology
CRISPR-Cas9
RELA
PIK3CA
BRCA2
Gene isoform
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