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High-efficiency cellular reprogramming with microfluidics
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DOI：10.1038/NMETH.3832.png)
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We report that the efficiency of reprogramming human somatic cells to induced pluripotent stem cells (hiPSCs) can be dramatically improved in a microfluidic environment. Microliter-volume confinement resulted in a 50-fold increase in efficiency over traditional reprogramming by delivery of synthetic mRNRNAs encoding transcription factors. In these small volumes, extracellular components of the TGF-beta and other signaling pathways exhibited temporal regulation that appears critical to acquisition of pluripotency. The high quality and purity of the resulting hiPSCs (mu-hiPSCs) allowed direct differentiation into functional hepatocyte-and cardiomyocyte-like cells in the same platform without additional expansion.
Keyword:
PLURIPOTENT STEM-CELLS
SOMATIC-CELLS
DIFFERENTIATION
INDUCTION
REVEALS
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