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Optimal Drift Correction for Superresolution Localization Microscopy with Bayesian Inference
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DOI：10.1016/j.bpj.2015.09.017.png)
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Single-molecule-localization-based superresolution microscopy requires accurate sample drift correction to achieve good results. Common approaches for drift compensation include using fiducial markers and direct drift estimation by image correlation. The former increases the experimental complexity and the latter estimates drift at a reduced temporal resolution. Here, we present, to our knowledge, a new approach for drift correction based on the Bayesian statistical framework. The technique has the advantage of being able to calculate the drifts for every image frame of the data set directly from the single-molecule coordinates. We present the theoretical foundation of the algorithm and an implementation that achieves significantly higher accuracy than image-correlation-based estimations.
Keyword:
CRK
ALGORITHM
PROTEINS
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期刊
IF:
3.1
论文数:
5.0W
被引数:
4.4W
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Localization events-based sample drift correction for localization microscopy with redundant cross-correlation algorithm.png)
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