Pooled genetic screens with image-based profiling

Russell T. Walton

doi:10.15252/msb.202110768

2022-11-11

Russell T. Walton

Avtar Singh

Paul C. Blainey *

DOI：10.15252/msb.202110768

原文链接

查看原文

摘要

En 中文

Spatial structure in biology, spanning molecular, organellular, cellular, tissue, and organismal scales, is encoded through a combination of genetic and epigenetic factors in individual cells. Microscopy remains the most direct approach to exploring the intricate spatial complexity defining biological systems and the structured dynamic responses of these systems to perturbations. Genetic screens with deep single-cell profiling via image features or gene expression programs have the capacity to show how biological systems work in detail by cataloging many cellular phenotypes with one experimental assay. Microscopy-based cellular profiling provides information complementary to next-generation sequencing (NGS) profiling and has only recently become compatible with large-scale genetic screens. Optical screening now offers the scale needed for systematic characterization and is poised for further scale-up. We discuss how these methodologies, together with emerging technologies for genetic perturbation and microscopy-based multiplexed molecular phenotyping, are powering new approaches to reveal genotype-phenotype relationships.

Keyword:

morphological profiling

multiplexed imaging

optical microscopy profiling

optical screening

pooled genetic screening

AI总结

对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。

期刊

Molecular Systems Biology

IF:

7.7

论文数:

1.6K

被引数:

1.0W

机构

Harvard University

学者数:

26.5W

论文数: 22.0W

被引数: 28.7W

引用论文

Fast beam searching concept for indoor Terahertz communications

The 8th European Conference on Antennas and Propagation (EuCAP 2014)

IF0

2014-04-01

PREAI

Bile Peng; Sebastian Priebe; Thomas Kurner

Spectral Efficiency Analysis of Uplink-Downlink Decoupled Access in C-V2X Networks C-V2X网络上下行解耦接入的频谱效率分析

GLOBECOM 2022 - 2022 IEEE Global Communications Conference

IF0

2022-12-04

OAAI

Luofang Jiao; Kai Yu; Yunting Xu; Tianqi Zhang; Haibo Zhou; Xuemin Shen

Video recognition using ambient light sensors

2016 IEEE International Conference on Pervasive Computing and Communications (PerCom)

IF0

2016-03-01

PREAI

Lorenz Schwittmann; Viktor Matkovic; Matthaus Wander; Torben Weis

APOE‐targeted epigenome therapy for late onset Alzheimer’s disease

Alzheimer's & Dementia

IF0

2022-12-20

PREAI

Boris Kantor; Joseph Rittiner; Peter J Nicholls; Ornit Chiba‐Falek

The effects of oxygen contamination on the properties of reactively sputtered indium nitride films

Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films

IF0

1988-05-01

PREAI

K. L. Westra; R. P. W. Lawson; M. J. Brett

L-Tryptophan-kynurenine pathway enzymes are therapeutic target for neuropsychiatric diseases: Focus on cell type differences

Neuropharmacology

IF0

2017-01-01

PREAI

Hidetsugu Fujigaki; Yasuko Yamamoto; Kuniaki Saito

Spatial organization of the somatosensory cortex revealed by osmFISH osmFISH揭示的体感皮层的空间组织

NATURE METHODS

IF32.1

2018-10-30

394

PREAI

Codeluppi, Simone; Borm, Lars E.; Zeisel, Amit; La Manno, Gioele; van Lunteren, Josina A.; Svensson, Camilla I.; Linnarsson, Sten

High-throughput functional genomics using CRISPR-Cas9 使用CRISPR-Cas9的高通量功能基因组学

NATURE REVIEWS GENETICS

IF52

2015-04-09

1.1K

OAAI

Shalem, Ophir; Sanjana, Neville E.; Zhang, Feng

One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes

MOLECULAR THERAPY-NUCLEIC ACIDS

IF0

2017-09-01

OAAI

Li, Shenglan; Zhang, Anqi; Xue, Haipeng; Li, Dali; Liu, Ying

High-throughput identification of genotype-specific cancer vulnerabilities in mixtures of barcoded tumor cell lines

NATURE BIOTECHNOLOGY

IF41.7

2016-02-29

255

OAAI

Yu, Channing; Mannan, Aristotle M.; Yvone, Griselda Metta; Ross, Kenneth N.; Zhang, Yan-Ling; Marton, Melissa A.; Taylor, Bradley R.; Crenshaw, Andrew; Gould, Joshua Z.; Tamayo, Pablo; Weir, Barbara A.; Tsherniak, Aviad; Wong, Bang; Garraway, Levi A.; Shamji, Alykhan F.; Palmer, Michelle A.; Foley, Michael A.; Winckler, Wendy; Schreiber, Stuart L.; Kung, Andrew L.; Golub, Todd R.

查看更多内容