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Simultaneous Multicolor Multifocal Scanning Microscopy
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DOI：10.1021/acsphotonics.3c00205.png)
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Super-resolution fluorescence microscopy has revolutionizedcellbiology over the past decade, enabling the visualization of subcellularcomplexity with unparalleled clarity and detail. However, the rapiddevelopment of image-scanning-based super-resolution systems stillrestrains convenient access to commonly used instruments such as epi-fluorescencemicroscopes. Here, we present multifocal scanning microscopy (MSM)for super-resolution imaging with simultaneous multicolor acquisitionand minimal instrumental complexity. MSM implements a stationary,interposed multifocal multicolor excitation by exploiting the motionof the specimens, realizing super-resolution microscopy through ageneral epi-fluorescence platform without compromising the image-scanningmechanism or inducing complex instrument alignment. The system isdemonstrated with various phantom and biological specimens, and theresults present effective resolution doubling, optical sectioning,and contrast enhancement. We anticipate MSM, as a highly accessibleand compatible super-resolution technique, to offer a promising methodologicalpathway for broad cell biological discoveries.
Keyword:
MSM
super-resolution systems
optical sectioning
super-resolution imaging
image-scanning microscopy
cell biology
期刊
IF:
6.7
论文数:
5.6K
被引数:
2.5W
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