SpaNorm: spatially-aware normalization for spatial transcriptomics data

doi:10.1186/s13059-025-03565-y

2025-04-29

DOI：10.1186/s13059-025-03565-y

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由于区域特异性文库大小与生物学之间的空间关联，空间转录组学数据的归一化具有挑战性。我们开发了SpaNorm，这是第一种具有空间感知的归一化方法，它同时建模文库大小效应和潜在生物学，分离这些效应，从而在不移除生物信息的情况下移除文库大小效应。使用来自4个技术平台的6个数据集的27个组织样本，SpaNorm在保留空间域信息并检测空间变异基因的同时，优于常用的单细胞归一化方法。SpaNorm具有通用性，适用于多细胞和亚细胞空间转录组学数据，且在不同分割方法下表现出相对稳健的性能。

Keyword:

SINGLE-CELL

ATLAS

期刊

Genome Biology

IF:

9.4

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6.4K

被引数:

7.3W

机构

Univ Sydney

学者数:

2.7K

论文数: 1.8K

被引数: 583

childrens med res inst

学者数:

论文数: 9

被引数: 3

Walter and Eliza Hall Institute of Medical Research

学者数:

671

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被引数: 1.0W

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