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SpaceBar enables single-cell-resolution clone tracing with imaging-based spatial transcriptomics
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DOI：10.1038/s41592-025-02968-w.png)
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Abstract
En 中文
Imaging-based spatial transcriptomics methods allow for the measurement of spatial determinants of cellular phenotypes but are incompatible with random barcode-based clone-tracing methods, preventing the simultaneous detection of clonal and spatial drivers. Here we report SpaceBar, which enables simultaneous clone tracing and spatial gene expression profiling with standard imaging-based spatial transcriptomics pipelines. Our approach uses a library of 96 synthetic barcode sequences that combinatorially labels each cell. Thus, SpaceBar can distinguish between clonal dynamics and environmentally driven transcriptional regulation in complex tissue contexts. SpaceBar is a cellular barcoding strategy for simultaneous analysis of cell clonal and spatial identities.
Keywords:
SpaceBar
clone tracing
spatial transcriptomics
cellular barcoding
gene expression profiling
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