Digital transcriptome profiling using selective hexamer priming for cDNA synthesis

doi:10.1038/NMETH.1360

2009-08-09

157

PRE

Christopher D. Armour *

Christopher K. Raymond

DOI：10.1038/NMETH.1360

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摘要

En 中文

We developed a procedure for the preparation of whole transcriptome cDNA libraries depleted of ribosomal RNA from only 1 mu g of total RNA. The method relies on a collection of short, computationally selected oligonucleotides, called 'not-so-random' (NSR) primers, to obtain full-length, strand-specific representation of nonribosomal RNA transcripts. In this study we validated the technique by profiling human whole brain and universal human reference RNA using ultra-high-throughput sequencing.

Keyword:

RNA

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期刊

Nature Methods

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32.1

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7.2K

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机构

merck & company usa

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