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Directed evolution in mammalian cells
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DOI：10.1038/s41592-021-01090-x.png)
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摘要
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Directed evolution experiments are typically carried out using in vitro systems, bacteria, or yeast-even when the goal is to probe or modulate mammalian biology. Performing directed evolution in systems that do not match the intended mammalian environment severely constrains the scope and functionality of the targets that can be evolved. We review new platforms that are now making it possible to use the mammalian cell itself as the setting for directed evolution and present an overview of frontier challenges and high-impact targets for this approach.
Keyword:
SOMATIC HYPERMUTATION
TRANSCRIPTION FACTORS
GENETIC-CODE
GENOMIC DNA
MUTAGENESIS
RESISTANCE
EFFICIENT
EXPRESSION
DISCOVERY
THERAPY
期刊
IF:
32.1
论文数:
7.2K
被引数:
12.7W
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