Efficient genome editing in zebrafish using a CRISPR-Cas system

doi:10.1038/nbt.2501

2013-01-29

3.1K

DOI：10.1038/nbt.2501

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摘要

En 中文

In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR) CRISPR-associated (Gas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator like effector nucleases.

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期刊

Nature Biotechnology

IF:

41.7

论文数:

1.2W

被引数:

10.1W

机构

Massachusetts General Hospital

学者数:

3.4W

论文数: 2.6W

被引数: 8.6W

Harvard University

学者数:

26.5W

论文数: 22.0W

被引数: 28.7W

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