.png){kind=icon}
返回
Nuisance compounds in cellular assays
.png){kind=icon}
OA
AI
DOI：10.1016/j.chembiol.2021.01.021.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Compounds that exhibit assay interference or undesirable mechanisms of bioactivity (nuisance compounds'') are routinely encountered in cellular assays, including phenotypic and high-content screening assays. Much is known regarding compound-dependent assay interferences in cell-free assays. However, despite the essential role of cellular assays in chemical biology and drug discovery, there is considerably less known about nuisance compounds in more complex cell-based assays. In our view, a major obstacle to realizing the full potential of chemical biology will not just be difficult-to-drug targets or even the sheer number of targets, but rather nuisance compounds, due to their ability to waste significant resources and erode scientific trust. In this review, we summarize our collective academic, government, and industry experiences regarding cellular nuisance compounds. We describe assay design strategies to mitigate the impact of nuisance compounds and suggest best practices to efficiently address these compounds in complex biological settings.
Keyword:
HIGH-THROUGHPUT IDENTIFICATION
SMALL-MOLECULE
NATURAL-PRODUCTS
CANCER-CELLS
DRUG
INTERFERENCE
INHIBITION
DISCOVERY
STABILITY
TARGETS
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
7.2
论文数:
1.8K
被引数:
9.1K
机构
查看更多机构
引用论文
High-throughput identification of genotype-specific cancer vulnerabilities in mixtures of barcoded tumor cell lines.png)
NATURE BIOTECHNOLOGYOAAI
NATURE BIOTECHNOLOGYIF41.7
OAAI分享收藏DRUG-seq for miniaturized high-throughput transcriptome profiling in drug discoveryNATURE COMMUNICATIONSOAAI
NATURE COMMUNICATIONSIF15.7
OAAI分享收藏 查看更多内容